A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
The major drawback of using Fluo-4 AM is that it requires an organic anion transporter inhibitor, such as probenecid, to prevent leakage.This can hinder the studies that require extended monitoring time and longer cellular retention.To address the issue, a novel Ca2+ indicator, Calbryte 520 AM, was developed.We compared the performance of Fluo-4 AM